Journal: Biomedical Optics Express
Article Title: Laser speckle contrast imaging of hepatic microcirculation
doi: 10.1364/BOE.554663
Figure Lengend Snippet: Experimental setup for imaging the hepatic microvasculature in vivo . a ) Rat preparation viewed from above. Images were acquired from a region (blue rectangle) of the left lateral lobe of the liver. b ) A bright-field image of the liver surface vasculature: terminal central venule (tCV), terminal portal venule (tPV), and sinusoids. c ) Laser speckle imaging setup. The liver surface was illuminated with a 785 nm laser diode; the scattered light was captured by a CMOS camera (see Methods). d ) Raw images were first stabilized by matching the position of the motion marker. The temporal laser speckle contrast and the temporal blood flow index (tBFI) were calculated from the stabilized images. tBFI was averaged over time to obtain tBFI maps. e ) A tBFI map showing hepatic microvessels. See Fig. S1 for a tBFI map obtained from a larger region of the liver. f,g ) Close-up of the terminal central venules (f) and sinusoids (g). See Fig. S2 showing sinusoids in another rat.
Article Snippet: The scattered light passed through a linear polarizing filter and was captured by a CMOS camera (Basler acA2048-90umNIR, 5.5 μ m pixel size, 8-bit mode).
Techniques: Imaging, In Vivo, Marker